Journal: BMC cancer

Article Title: Suppressing Dazl modulates tumorigenicity and stemness in human glioblastoma cells.

doi: 10.1186/s12885-020-07155-y

Figure Lengend Snippet: Fig. 2 Dazl knockdown inhibited the proliferation and germline traits of glioblastoma cells in vitro. a Western blot analysis detected whether Cas9 protein was transfected into GBM cells successfully and whether Dazl protein was deleted. b A CCK-8 cell proliferation assay was performed after Dazl deletion in A172, U251, and LN229 cells. c An alkaline phosphatase stain assay was performed between the WT GBM cell lines and the Dazl deletion cells. Images were taken from the inverted microscope (magnification × 200). All experiments were carried out in triplicate. Data are shown as the mean ± SE (*P < 0.05, **P < 0.01)

Article Snippet: GBM cells were transfected with CRISPR plasmids and the lenti-cas9 pSpCas9(BB)-2A-GFP (PX458) plasmid (Addgene plasmid #48138) using XtremeGENE 9 DNA Transfection Reagent (6,365,787, 001, Sigma-Aldrich, USA).

Techniques: Knockdown, In Vitro, Western Blot, Transfection, CCK-8 Assay, Proliferation Assay, Staining, Inverted Microscopy